Proteomic Challenges

 

 

                

 

 

Proteome and Proteomics challenges to date

The completion of the human genome sequence more than a decade ago was an indisputable triumph for biomedical research; however, it did not provide answers it promised to tackle diseases which have baffled mankind. Since then, researchers have continued to expand their knowledge of basic elements within the genome, where connecting genotype to phenotype will require an ‘in-depth’ view of how the protein products of genes operate and interact.  The prevalent view in the past was that flow of exciting information would be from genomic breakthroughs to transcriptome to proteome. However, very soon it become apparent that proteome and proteomics can be used to annotate the genome and that the genomics field can now hugely benefit from proteomics research. 

 

Despite excellent achievements over the decade, there remained a “long gap” between primary genetic information which was rather static and easy to read, to the ultimate form of real information reflected in the function(s) of the protein coded by a given gene where proteins carry out these functionalities to drive biological processes in response to variety of stimulus. Late 1970’s witnessed development of various analytical methods allowing fractionation of hundreds of diverse proteins at once.  It is not surprising that both Genomic and Proteomic progresses are very closely linked to the development of novel technologies and methods. 

 

The term “proteome” was introduced at the 1st Siena Conference in 1994 in a presentation  entitled “From Genome to Proteome”. Since then, the words Proteome and Proteomics have become representatives of complex approach to the study of the proteins (a fitting analogy to the term genomics).  Over the years, Proteomics went through the various time periods including awareness of its own potential and fascination by emerging complexity on one side, on the other side it suffered from the lack of means for protein identity. Luckily, this was resolved by the emergence of Edman sequencing followed by mass spectrometry focus on protein entity.   Of the many proteomic technologies current focus appears to be on the development of mass spectrometers, nano-chromatographic systems and robust bio-informatic tools. Many of the studies identified around ten of thousands of protein products of gene expression at once, but with little interest to the biological meaning and ability to distinguish  possible  individual variants of the protein  (protein species/proteoforms)   due to  mainly posttraslational  modifications  and RNA splicing.  While large proteomic data sets have been collected from many different tissue types, more needs to be done and efforts need to be directed towards tissue-specific proteins and posttranslational modifications that affect protein-protein interactions, in addition to protein functionality.

 

Proteomics linking Genomics and Systems Biology

The greatest promise for the detection and treatment of diseases lies in the deep understanding of molecular basis for disease initiation, progression and efficacious treatment based on the discovery of unique biomarkers. Although progress in genomics has been rapid during the past few years, it only provides us with a glimpse of what may occur as dictated by the genetic code. In reality, we still need to measure what is happening in a patient in real time, which means finding tell-tale proteins that provide insight into the biological processes of disease development. This is because genes are only the "recipes" of the cell, while the proteins encoded by the genes are ultimately the functional players that drive both normal and disease physiology.

 

                    

                 Genomics                                                         Proteomics                                                  Metabolomics

 

Proteomics urgencies:

  • Comprehensive analytical proteomic technologies  to deal with  more than 106 dynamic protein range in tissues and body fluids
  • Improvements in quantitative proteomics in order to eliminate variability in protein detection and quantification leading to analytical variability which is not relevat to biological system
  • Protein interaction studies to decipher biological function of the proteins
  • Bioinformatic algorithms  allowing data reduction, evaluation  and their conversion to biological usefulness
  • Standardisation of methodologies, assays and information
  • Sensitivity, reproducibility and robustness of assays and technologies
  • Targeted proteomics - good assay development and  biomarker  validation
  • Integration between target identification and product development in pharmaceutical research
  • Challenges are also in the translation of proteomic research to medicine, food industry, and environment (plant research, microbial, bacterial, fisheries, etc.).
  • Medical research is vital for improving human health as it brings about a better understanding of the cause and effect of complex diseases. Promoting innovative medical research and its clinical application towards improved human health is absolutely vital as we progress

 

 

 

Promoting societal needs............our ageing population and their urgent needs 

 

                          

 

                                                                                                                                                              

                                                                                                                                                Data - 2015

 

 

Aging issues as of now

Aging is associated with a wide range of human disorders, including cancer, diabetes, cardiovascular, and neurodegenerative diseases.  Long thought to be an inexorable road toward decline and diseases, the aging process needs 'in-depth' understanding. Although many studies have focused on the genes that impact aging, the nongenetic regulation of aging and proteogenomic changes need careful examination.  The potential reversibility of these epigenetic and proteomic changes that occur as a hallmark of aging offer exciting opportunities to alter the trajectory of age-related diseases and / or impact quality of life.

 

Aging issues in ten years from now !

By 2030, it is estimated that approximately 25 percent of the Europe’s population will have had their 65th birthday.  We know that aging is not just a European problem but a global issue.  Additionally, World Health Organization (WHO) estimates that the proportion of deaths worldwide caused by chronic diseases will rise from 59 percent in 2002 to 69 percent in 2030.  Some infectious diseases too are increasing in frequency, and the combined burden of chronic and infectious disease is predicted to be an increasingly significant issue for public healthcare systems and clinicians, researchers and scientists.

 

Aging is not all doom and gloom!

Whilst addressing aging, we should also enjoy 'healthy biological aging'

                                      which goes 'hand in hand' with ...........

                                                                                    a good aged and mature wine!

                                                                                                       & lets be honest.............!

                                                                                                                            .... n ˈviːnəʊ ˈvɛrɪˌtæs

                                     

 

                                                in vino veritas   -   (ɪn ˈviːnəʊ ˈvɛrɪˌtæs)​

 

 

            
        Proteome and Proteomics addressing societal challenges of the decade to come !